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pcag rfp int vector  (Addgene inc)


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    Addgene inc pcag rfp int vector
    Pcag Rfp Int Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+rfp+int+vector/pCAG-RFP-int+(Plasmid+%2319822)/pmc03861205-323-25-27
    Average 93 stars, based on 7 article reviews
    pcag rfp int vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Luciferase:

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. Luciferase Assays The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. The region of each target gene predicted to contain a mmu-miR-138-1 seed match (identified in TargetScan ( Lewis et al., 2005 ), microCosm ( Griffiths-Jones et al., 2008 ), or in the case of Sox2, the rna22 algorithm ( Miranda et al., 2006 ) identified two sites in the coding sequence as previously described ( Tay et al., 2008 )) was PCR purified from genomic DNA using primers including XbaI restriction sites ( Fig S1 ).

    Sequencing:

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. Luciferase Assays The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. The region of each target gene predicted to contain a mmu-miR-138-1 seed match (identified in TargetScan ( Lewis et al., 2005 ), microCosm ( Griffiths-Jones et al., 2008 ), or in the case of Sox2, the rna22 algorithm ( Miranda et al., 2006 ) identified two sites in the coding sequence as previously described ( Tay et al., 2008 )) was PCR purified from genomic DNA using primers including XbaI restriction sites ( Fig S1 ).

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1 /Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: MetaMorph Software (Molecular Devices) was used to quantify Lmx1a, Lmx1b, and Nkx6.1 levels in Foxa2+ cells of 15 embryoid bodies (5 each from 3 independent experiments, n = 3). .. The stem-loop of mmu-miR-135a-2 (accession MI0000715) plus ∼100 bp 5′ and 3′ flanking sequence was PCR purified from genomic DNA and then cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. A fragment of each target gene 3′UTR , which corresponds to the region predicted to contain a mmu-miR-135a-2 seed match, was PCR purified from genomic DNA and then cloned into the pmirGLO vector (Promega E1330).

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). ..

    Clone Assay:

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. Luciferase Assays The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. The region of each target gene predicted to contain a mmu-miR-138-1 seed match (identified in TargetScan ( Lewis et al., 2005 ), microCosm ( Griffiths-Jones et al., 2008 ), or in the case of Sox2, the rna22 algorithm ( Miranda et al., 2006 ) identified two sites in the coding sequence as previously described ( Tay et al., 2008 )) was PCR purified from genomic DNA using primers including XbaI restriction sites ( Fig S1 ).

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1 /Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: MetaMorph Software (Molecular Devices) was used to quantify Lmx1a, Lmx1b, and Nkx6.1 levels in Foxa2+ cells of 15 embryoid bodies (5 each from 3 independent experiments, n = 3). .. The stem-loop of mmu-miR-135a-2 (accession MI0000715) plus ∼100 bp 5′ and 3′ flanking sequence was PCR purified from genomic DNA and then cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. A fragment of each target gene 3′UTR , which corresponds to the region predicted to contain a mmu-miR-135a-2 seed match, was PCR purified from genomic DNA and then cloned into the pmirGLO vector (Promega E1330).

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). ..

    Plasmid Preparation:

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. Luciferase Assays The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. The region of each target gene predicted to contain a mmu-miR-138-1 seed match (identified in TargetScan ( Lewis et al., 2005 ), microCosm ( Griffiths-Jones et al., 2008 ), or in the case of Sox2, the rna22 algorithm ( Miranda et al., 2006 ) identified two sites in the coding sequence as previously described ( Tay et al., 2008 )) was PCR purified from genomic DNA using primers including XbaI restriction sites ( Fig S1 ).

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1 /Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: MetaMorph Software (Molecular Devices) was used to quantify Lmx1a, Lmx1b, and Nkx6.1 levels in Foxa2+ cells of 15 embryoid bodies (5 each from 3 independent experiments, n = 3). .. The stem-loop of mmu-miR-135a-2 (accession MI0000715) plus ∼100 bp 5′ and 3′ flanking sequence was PCR purified from genomic DNA and then cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. A fragment of each target gene 3′UTR , which corresponds to the region predicted to contain a mmu-miR-135a-2 seed match, was PCR purified from genomic DNA and then cloned into the pmirGLO vector (Promega E1330).

    Article Title: MicroRNA deficient Schwann cells display congenital hypomyelination
    Article Snippet: .. The stem-loop of mmu-miR-138-1 (accession MI0000722) plus ~100 bp 5′ and 3′ flanking sequence was cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). ..

    Polymerase Chain Reaction:

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1 /Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: MetaMorph Software (Molecular Devices) was used to quantify Lmx1a, Lmx1b, and Nkx6.1 levels in Foxa2+ cells of 15 embryoid bodies (5 each from 3 independent experiments, n = 3). .. The stem-loop of mmu-miR-135a-2 (accession MI0000715) plus ∼100 bp 5′ and 3′ flanking sequence was PCR purified from genomic DNA and then cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. A fragment of each target gene 3′UTR , which corresponds to the region predicted to contain a mmu-miR-135a-2 seed match, was PCR purified from genomic DNA and then cloned into the pmirGLO vector (Promega E1330).

    Purification:

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1 /Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: MetaMorph Software (Molecular Devices) was used to quantify Lmx1a, Lmx1b, and Nkx6.1 levels in Foxa2+ cells of 15 embryoid bodies (5 each from 3 independent experiments, n = 3). .. The stem-loop of mmu-miR-135a-2 (accession MI0000715) plus ∼100 bp 5′ and 3′ flanking sequence was PCR purified from genomic DNA and then cloned into the pCAG-RFP-int vector (Addgene plasmid 19822). .. A fragment of each target gene 3′UTR , which corresponds to the region predicted to contain a mmu-miR-135a-2 seed match, was PCR purified from genomic DNA and then cloned into the pmirGLO vector (Promega E1330).



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    pcag rfp int vector  (Addgene inc)
    93
    Addgene inc pcag rfp int vector
    Pcag Rfp Int Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+rfp+int+vector/pCAG-RFP-int+(Plasmid+%2319822)/pmc03861205-323-25-27
    Average 93 stars, based on 1 article reviews
    pcag rfp int vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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